GTX105255

antibody from GeneTex

Targeting: PPP1CA PP-1A, PP1A, PP1alpha, PPP1A

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX105255

Provider

GeneTex

Proper citation

GeneTex Cat#GTX105255, RRID:AB_1951346

Product name

PPP1A antibody

Antibody type

Polyclonal

Reactivity

Human, Rat

Host

Rabbit

References

Submitted references

Carfilzomib induces leukaemia cell apoptosis via inhibiting ELK1/KIAA1524 (Elk-1/CIP2A) and activating PP2A not related to proteasome inhibition.

Liu CY, Hsieh FS, Chu PY, Tsai WC, Huang CT, Yu YB, Huang TT, Ko PS, Hung MH, Wang WL, Shiau CW, Chen KF
British journal of haematology 2017 Jun;177(5):726-740

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Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.

Wei R, Ngo B, Wu G, Lee WH
Molecular biology of the cell 2011 Oct;22(19):3584-94

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using GTX105255 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

http://www.genetex.com

KD Reagent Prod no

GTX105255

KD Reagent Provider

Customized siRNA

Antibody Lot Number

39932

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ?g of whole cell lysate)A:293TB:A431(GTX27909)12% SDS PAGEGTX105255 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PPP1A antibody detects PPP1A protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A(GTX105255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A(GTX105255) antibody at 1:100 dilution.