Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB1112 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1112, RRID:AB_1579002
- Product name
- PPP1CA monoclonal antibody, clone 4G3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant PPP1CA.
- Isotype
- IgG
- Antibody clone number
- 4G3
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Gene amplification and overexpression of protein phosphatase 1alpha in oral squamous cell carcinoma cell lines.
Protein phosphatase 1--targeted in many directions.
Hsu LC, Huang X, Seasholtz S, Potter DM, Gollin SM
Oncogene 2006 Sep 7;25(40):5517-26
Oncogene 2006 Sep 7;25(40):5517-26
Protein phosphatase 1--targeted in many directions.
Cohen PT
Journal of cell science 2002 Jan 15;115(Pt 2):241-56
Journal of cell science 2002 Jan 15;115(Pt 2):241-56
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of cell lysates of HepG2 (45 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with PPP1CA monoclonal antibody , clone 4G3 (1 : 1000) (Cat # MAB1112). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.