M02801

antibody from Boster Biological Technology

Targeting: PPP1CA PP-1A, PP1A, PP1alpha, PPP1A

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M02801

Provider

Boster Biological Technology

Product name

Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for PP1/PPP1CA detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat PP1/PPP1CA information: Molecular Weight: 37512 MW; Subcellular Localization: Cytoplasm. Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AFC-16

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human pancreas, using PPP1CA+PPP1CB Antibody(M02801)PPP1CA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PPP1CA Antibody (M02801)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image