Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB6105 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PP1 alpha Catalytic Subunit Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human PP1 alpha Catalytic Subunit in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) PP1 beta or rhPP1 gamma is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P62136- Isotype
- IgG
- Antibody clone number
- 490314
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.
Cervero P, Wiesner C, Bouissou A, Poincloux R, Linder S
Nature communications 2018 Feb 6;9(1):515
Nature communications 2018 Feb 6;9(1):515
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PP1 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CHP-100 human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PP1 alpha Catalytic Subunit Monoclonal Antibody (Catalog # MAB6105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1 alpha , recombinant human PP1 beta , and recombinant human PP1 gamma (5 ng/lane) were included. A specific band was detected for PP1 alpha at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
