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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- 14326-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RHOB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAIRKKLV VVGDGACGKT CLLIVFSKDE FPEVYVPTVF ENYVADIEVD GKQVELALWD TAGQEDYDRL RPLSYPDTDV ILMCFSVDSP DSLENIPEKW VPEVKHFCPN VPIILVANKK DLRSDEHVRT ELARMKQEPV RTDDGRAMAV RIQAYDYLEC SAKTKEGVRE VFETATRAAL QKRYGSQNGC INCCKVL (1-196 aa encoded by BC066954)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 14326-1-AP ( RHOB antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 14326-1-AP ( RHOB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RHOB (IP:14326-1-AP, 3ug; Detection:14326-1-AP 1:2000) with HeLa cells lysate 2800ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RHOB (IP:14326-1-AP, 4 ug; Detection:14326-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.
