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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX109665 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109665, RRID:AB_1951580
- Product name
- RAB5A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX109665 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A:A431(GTX27909)B:Raji (GTX27908)12% SDS PAGEGTX109665 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rab5A antibody immunoprecipitates Rab5A protein in IP experiments. IP Sample: 1 mg HeLa whole cell lysate/extract A. 30 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of Rab5A protein by 2 £gg of Rab5A antibody (GTX109665) 15% SDS-PAGE The immunoprecipitated Rab5A protein was detected by Rab5A antibody (GTX109665) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB5A antibody detects RAB5A protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. RAB5A antibody (GTX109665) dilution: 1:500.
