Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-05958 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05958, RRID:AB_1555346
- Product name
- Rabbit Polyclonal SOS2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOS2 Antibody [NBP1-05958] - Detection of Human and Mouse SOS2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOS2 antibody NBP1-05958 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOS2 Antibody [NBP1-05958] - Samples: Whole cell lysate from HeLa and 293T cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SOS2 Antibody [NBP1-05958] - Detection of human SOS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOS2 antibody NBP1-05958 (lot NBP1-05958-2) used for IP at 6 ug per reaction. SOS2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05958-1). For blotting immunoprecipitated SOS2, NBP1-05958 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.