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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8409 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8409, RRID:AB_628391
- Product name
- Anti-TRAF6
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references p75 neurotrophin receptor-mediated apoptosis in sympathetic neurons involves a biphasic activation of JNK and up-regulation of tumor necrosis factor-alpha-converting enzyme/ADAM17.
The protein kinase C-responsive inhibitory domain of CARD11 functions in NF-kappaB activation to regulate the association of multiple signaling cofactors that differentially depend on Bcl10 and MALT1 for association.
Induction of proneurotrophins and activation of p75NTR-mediated apoptosis via neurotrophin receptor-interacting factor in hippocampal neurons after seizures.
The type I TGF-β receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner
Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing.
Kenchappa RS, Tep C, Korade Z, Urra S, Bronfman FC, Yoon SO, Carter BD
The Journal of biological chemistry 2010 Jun 25;285(26):20358-68
The Journal of biological chemistry 2010 Jun 25;285(26):20358-68
The protein kinase C-responsive inhibitory domain of CARD11 functions in NF-kappaB activation to regulate the association of multiple signaling cofactors that differentially depend on Bcl10 and MALT1 for association.
McCully RR, Pomerantz JL
Molecular and cellular biology 2008 Sep;28(18):5668-86
Molecular and cellular biology 2008 Sep;28(18):5668-86
Induction of proneurotrophins and activation of p75NTR-mediated apoptosis via neurotrophin receptor-interacting factor in hippocampal neurons after seizures.
Volosin M, Trotter C, Cragnolini A, Kenchappa RS, Light M, Hempstead BL, Carter BD, Friedman WJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Sep 24;28(39):9870-9
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Sep 24;28(39):9870-9
The type I TGF-β receptor engages TRAF6 to activate TAK1 in a receptor kinase-independent manner
Alessandro Sorrentino, Noopur Thakur, Susanne Grimsby, Anders Marcusson, Verena von Bulow, Norbert Schuster, Shouting Zhang, Carl-Henrik Heldin, Maréne Landström
Nature Cell Biology 2008 Aug;10(10):1199-1207
Nature Cell Biology 2008 Aug;10(10):1199-1207
Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing.
Wang S, Shi Z, Liu W, Jules J, Feng X
BMC biotechnology 2006 Dec 22;6:50
BMC biotechnology 2006 Dec 22;6:50
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 60
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
