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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF1056 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat TrkA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human TrkA and less than 1% cross-reactivity with recombinant mouse (rm) TrkB and rmTrkC is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P35739- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis.
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis.
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration.
The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur.
Optogenetic Activation of Non-Nociceptive Aβ Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats.
Immunohistochemical localization of nerve growth factor, tropomyosin receptor kinase A, and p75 in the bone and articular cartilage of the mouse femur.
Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway.
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain.
An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Bartesaghi L, Wang Y, Fontanet P, Wanderoy S, Berger F, Wu H, Akkuratova N, Bouçanova F, Médard JJ, Petitpré C, Landy MA, Zhang MD, Harrer P, Stendel C, Stucka R, Dusl M, Kastriti ME, Croci L, Lai HC, Consalez GG, Pattyn A, Ernfors P, Senderek J, Adameyko I, Lallemend F, Hadjab S, Chrast R
Cell reports 2019 Mar 26;26(13):3484-3492.e4
Cell reports 2019 Mar 26;26(13):3484-3492.e4
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis.
Chen H, Hu B, Lv X, Zhu S, Zhen G, Wan M, Jain A, Gao B, Chai Y, Yang M, Wang X, Deng R, Wang L, Cao Y, Ni S, Liu S, Yuan W, Chen H, Dong X, Guan Y, Yang H, Cao X
Nature communications 2019 Jan 14;10(1):181
Nature communications 2019 Jan 14;10(1):181
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration.
Wiszniak S, Schwarz Q
BMC neuroscience 2019 Apr 29;20(1):21
BMC neuroscience 2019 Apr 29;20(1):21
The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur.
Chartier SR, Mitchell SAT, Majuta LA, Mantyh PW
Neuroscience 2018 Sep 1;387:178-190
Neuroscience 2018 Sep 1;387:178-190
Optogenetic Activation of Non-Nociceptive Aβ Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats.
Tashima R, Koga K, Sekine M, Kanehisa K, Kohro Y, Tominaga K, Matsushita K, Tozaki-Saitoh H, Fukazawa Y, Inoue K, Yawo H, Furue H, Tsuda M
eNeuro 2018 Jan-Feb;5(1)
eNeuro 2018 Jan-Feb;5(1)
Immunohistochemical localization of nerve growth factor, tropomyosin receptor kinase A, and p75 in the bone and articular cartilage of the mouse femur.
Chartier SR, Mitchell SA, Majuta LA, Mantyh PW
Molecular pain 2017 Jan-Dec;13:1744806917745465
Molecular pain 2017 Jan-Dec;13:1744806917745465
Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway.
Price EA, Krasowska-Zoladek A, Nanda KK, Stachel SJ, Henze DA
Journal of neuroscience methods 2017 Apr 15;282:34-42
Journal of neuroscience methods 2017 Apr 15;282:34-42
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Usoskin D, Furlan A, Islam S, Abdo H, Lönnerberg P, Lou D, Hjerling-Leffler J, Haeggström J, Kharchenko O, Kharchenko PV, Linnarsson S, Ernfors P
Nature neuroscience 2015 Jan;18(1):145-53
Nature neuroscience 2015 Jan;18(1):145-53
TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson SN
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jan 22;34(4):1494-509
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jan 22;34(4):1494-509
Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain.
Evans LJ, Loescher AR, Boissonade FM, Whawell SA, Robinson PP, Andrew D
BMC neuroscience 2014 Jan 1;15:1
BMC neuroscience 2014 Jan 1;15:1
An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
Matusica D, Skeldal S, Sykes AM, Palstra N, Sharma A, Coulson EJ
The Journal of biological chemistry 2013 Apr 19;288(16):11144-54
The Journal of biological chemistry 2013 Apr 19;288(16):11144-54
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Saygili E, Schauerte P, Küppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schröder JW, Marx N, Rana OR
Journal of molecular and cellular cardiology 2010 Jul;49(1):79-87
Journal of molecular and cellular cardiology 2010 Jul;49(1):79-87
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat TrkA by Western Blot. Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TrkA in Rat Dorsal Root Ganglion. TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
