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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-109216 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TrkA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SHSY5Y, SHG-44, PC-12, Human liver cancer tissue, human stomach cancer tissue, mouse brain tissue, rat brain tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
Submitted references microRNA-454-mediated NEDD4-2/TrkA/cAMP axis in heart failure: Mechanisms and cardioprotective implications.
Wang Y, Pan W, Bai X, Wang X, Wang Y, Yin Y
Journal of cellular and molecular medicine 2021 Jun;25(11):5082-5098
Journal of cellular and molecular medicine 2021 Jun;25(11):5082-5098
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TrkA in SHSY5Y (1) and SHG-44 (2) cell lysates. Samples were incubated with TrkA polyclonal antibody (Product # PA5-109216), at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TrkA in PC-12 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with TrkA polyclonal antibody (Product # PA5-109216), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TrkA in paraffin-embedded rat brain tissue. Samples were incubated with TrkA polyclonal antibody (Product # PA5-109216), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TrkA in paraffin-embedded mouse brain tissue. Samples were incubated with TrkA polyclonal antibody (Product # PA5-109216), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TrkA in paraffin-embedded human stomach cancer tissue. Samples were incubated with TrkA polyclonal antibody (Product # PA5-109216), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TrkA in paraffin-embedded human liver cancer tissue. Samples were incubated with TrkA polyclonal antibody (Product # PA5-109216), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TrkA in SHSY5Y cells (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Samples were incubated with TrkA polyclonal antibody (Product # PA5-109216) at a dilution of 1:100, followed by Goat anti rabbit IgG (FITC).
