PAB28532

antibody from Abnova Corporation

Targeting: SIX1 DFNA23

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28532

Provider

Abnova Corporation

Product name

SIX1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SIX1.

Antigen sequence

CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLT
TTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNK
QNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLL
QGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQD
S


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with SIX1 polyclonal antibody (Cat # PAB28532) at 1:100-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with SIX1 polyclonal antibody (Cat # PAB28532) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with SIX1 polyclonal antibody (Cat # PAB28532) at 1-4 ug/ml shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle with SIX1 polyclonal antibody (Cat # PAB28532) shows moderate nuclear positivity in myocytes at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)