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- References [16]
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- Product number
- NBP1-84264 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84264, RRID:AB_11005131
- Product name
- Rabbit Polyclonal SIX1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SIX1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes.
Aberrant expression of homeobox gene SIX1 in Hodgkin lymphoma.
Overexpression of SIX1 is an independent prognostic marker in stage I-III colorectal cancer.
The Six1 oncoprotein downregulates p53 via concomitant regulation of RPL26 and microRNA-27a-3p.
3D mouse embryonic stem cell culture for generating inner ear organoids.
Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma.
Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype.
Persistently altered epigenetic marks in the mouse uterus after neonatal estrogen exposure.
Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration.
SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation.
Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.
Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-β signaling, epithelial-mesenchymal transition, and cancer stem cell properties.
The miR-106b-25 cluster targets Smad7, activates TGF-β signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer.
The Six1 homeoprotein induces human mammary carcinoma cells to undergo epithelial-mesenchymal transition and metastasis in mice through increasing TGF-beta signaling.
Six1 expands the mouse mammary epithelial stem/progenitor cell pool and induces mammary tumors that undergo epithelial-mesenchymal transition.
Gene expression changes during HPV-mediated carcinogenesis: a comparison between an in vitro cell model and cervical cancer.
Adrados I, Larrasa-Alonso J, Galarreta A, López-Antona I, Menéndez C, Abad M, Gil J, Moreno-Bueno G, Palmero I
Oncogene 2016 Jul 7;35(27):3485-94
Oncogene 2016 Jul 7;35(27):3485-94
Aberrant expression of homeobox gene SIX1 in Hodgkin lymphoma.
Nagel S, Meyer C, Kaufmann M, Drexler HG, MacLeod RA
Oncotarget 2015 Nov 24;6(37):40112-26
Oncotarget 2015 Nov 24;6(37):40112-26
Overexpression of SIX1 is an independent prognostic marker in stage I-III colorectal cancer.
Kahlert C, Lerbs T, Pecqueux M, Herpel E, Hoffmeister M, Jansen L, Brenner H, Chang-Claude J, Bläker H, Kloor M, Roth W, Pilarsky C, Rahbari NN, Schölch S, Bork U, Reissfelder C, Weitz J, Aust D, Koch M
International journal of cancer 2015 Nov 1;137(9):2104-13
International journal of cancer 2015 Nov 1;137(9):2104-13
The Six1 oncoprotein downregulates p53 via concomitant regulation of RPL26 and microRNA-27a-3p.
Towers CG, Guarnieri AL, Micalizzi DS, Harrell JC, Gillen AE, Kim J, Wang CA, Oliphant MUJ, Drasin DJ, Guney MA, Kabos P, Sartorius CA, Tan AC, Perou CM, Espinosa JM, Ford HL
Nature communications 2015 Dec 21;6:10077
Nature communications 2015 Dec 21;6:10077
3D mouse embryonic stem cell culture for generating inner ear organoids.
Koehler KR, Hashino E
Nature protocols 2014;9(6):1229-44
Nature protocols 2014;9(6):1229-44
Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma.
Kong J, Zhou X, Liu S, Jin T, Piao Y, Liu C, Lin Z
International journal of clinical and experimental pathology 2014;7(6):3018-27
International journal of clinical and experimental pathology 2014;7(6):3018-27
Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype.
Sakakibara I, Santolini M, Ferry A, Hakim V, Maire P
PLoS genetics 2014;10(5):e1004386
PLoS genetics 2014;10(5):e1004386
Persistently altered epigenetic marks in the mouse uterus after neonatal estrogen exposure.
Jefferson WN, Chevalier DM, Phelps JY, Cantor AM, Padilla-Banks E, Newbold RR, Archer TK, Kinyamu HK, Williams CJ
Molecular endocrinology (Baltimore, Md.) 2013 Oct;27(10):1666-77
Molecular endocrinology (Baltimore, Md.) 2013 Oct;27(10):1666-77
Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration.
Le Grand F, Grifone R, Mourikis P, Houbron C, Gigaud C, Pujol J, Maillet M, Pagès G, Rudnicki M, Tajbakhsh S, Maire P
The Journal of cell biology 2012 Sep 3;198(5):815-32
The Journal of cell biology 2012 Sep 3;198(5):815-32
SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation.
Ono H, Imoto I, Kozaki K, Tsuda H, Matsui T, Kurasawa Y, Muramatsu T, Sugihara K, Inazawa J
Oncogene 2012 Nov 22;31(47):4923-34
Oncogene 2012 Nov 22;31(47):4923-34
Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways.
Iwanaga R, Wang CA, Micalizzi DS, Harrell JC, Jedlicka P, Sartorius CA, Kabos P, Farabaugh SM, Bradford AP, Ford HL
Breast cancer research : BCR 2012 Jul 5;14(4):R100
Breast cancer research : BCR 2012 Jul 5;14(4):R100
Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-β signaling, epithelial-mesenchymal transition, and cancer stem cell properties.
Farabaugh SM, Micalizzi DS, Jedlicka P, Zhao R, Ford HL
Oncogene 2012 Feb 2;31(5):552-62
Oncogene 2012 Feb 2;31(5):552-62
The miR-106b-25 cluster targets Smad7, activates TGF-β signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer.
Smith AL, Iwanaga R, Drasin DJ, Micalizzi DS, Vartuli RL, Tan AC, Ford HL
Oncogene 2012 Dec 13;31(50):5162-71
Oncogene 2012 Dec 13;31(50):5162-71
The Six1 homeoprotein induces human mammary carcinoma cells to undergo epithelial-mesenchymal transition and metastasis in mice through increasing TGF-beta signaling.
Micalizzi DS, Christensen KL, Jedlicka P, Coletta RD, Barón AE, Harrell JC, Horwitz KB, Billheimer D, Heichman KA, Welm AL, Schiemann WP, Ford HL
The Journal of clinical investigation 2009 Sep;119(9):2678-90
The Journal of clinical investigation 2009 Sep;119(9):2678-90
Six1 expands the mouse mammary epithelial stem/progenitor cell pool and induces mammary tumors that undergo epithelial-mesenchymal transition.
McCoy EL, Iwanaga R, Jedlicka P, Abbey NS, Chodosh LA, Heichman KA, Welm AL, Ford HL
The Journal of clinical investigation 2009 Sep;119(9):2663-77
The Journal of clinical investigation 2009 Sep;119(9):2663-77
Gene expression changes during HPV-mediated carcinogenesis: a comparison between an in vitro cell model and cervical cancer.
Wan F, Miao X, Quraishi I, Kennedy V, Creek KE, Pirisi L
International journal of cancer 2008 Jul 1;123(1):32-40
International journal of cancer 2008 Jul 1;123(1):32-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in human cell line RH-30.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in mouse cell line NIH-3T3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in control (vector only transfected HEK293T lysate) and SIX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining in human skeletal muscle and liver tissues . Corresponding SIX1 RNA-seq data are presented for the same tissues.
