Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PAB9606 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9606, RRID:AB_1676186
- Product name
- ALOX5 (phospho S523) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ALOX5.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523.
Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis.
Prostaglandins and leukotrienes: advances in eicosanoid biology.
Luo M, Jones SM, Phare SM, Coffey MJ, Peters-Golden M, Brock TG
The Journal of biological chemistry 2004 Oct 1;279(40):41512-20
The Journal of biological chemistry 2004 Oct 1;279(40):41512-20
Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis.
Dwyer JH, Allayee H, Dwyer KM, Fan J, Wu H, Mar R, Lusis AJ, Mehrabian M
The New England journal of medicine 2004 Jan 1;350(1):29-37
The New England journal of medicine 2004 Jan 1;350(1):29-37
Prostaglandins and leukotrienes: advances in eicosanoid biology.
Funk CD
Science (New York, N.Y.) 2001 Nov 30;294(5548):1871-5
Science (New York, N.Y.) 2001 Nov 30;294(5548):1871-5
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of ALOX5 phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the ALOX5 (phospho S523) polyclonal antibody (Cat # PAB9606). The immunolabeling is completely eliminated by treatment with lambda-Ptase.