Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- PA5-78762 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALOX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references ALOX5-5-HETE promotes gastric cancer growth and alleviates chemotherapy toxicity via MEK/ERK activation.
Tang J, Zhang C, Lin J, Duan P, Long J, Zhu H
Cancer medicine 2021 Aug;10(15):5246-5255
Cancer medicine 2021 Aug;10(15):5246-5255
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX5 in SGC whole cell lysate using 40 µg per well. Sample was incubated with ALOX5 (Product # PA5-78762) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALOX5 in SGC whole cell lysate using 40 µg per well. Sample was incubated with ALOX5 (Product # PA5-78762) at a dilution of 0.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ALOX5 on paraffin-embedded human intestinal cancer tissue. Sample was incubated with ALOX5 polyclonal antibody (Product# PA5-78762).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ALOX5 on paraffin-embedded human mammary cancer tissue. Sample was incubated with ALOX5 polyclonal antibody (Product# PA5-78762).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ALOX5 on paraffin-embedded rat brain tissue. Sample was incubated with ALOX5 polyclonal antibody (Product# PA5-78762).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ALOX5 on paraffin-embedded mouse brain tissue. Sample was incubated with ALOX5 polyclonal antibody (Product# PA5-78762).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 1 Alox5-5-HETE axis is upregulated in gastric cancer tissues. (A) Scatter plot of Alox5 protein level in paired normal and tumor tissues obtained from 52 patients with gastric tumor. (B) Representative immunohistochemistry analysis of normal and tumor gastric tissues from patient#23 performed by Alox5 staining. (C) Scatter plot of 5-HETE level in paired normal and tumor tissues obtained from 52 patients with gastric tumor. Alox5 and 5-HETE were assessed using tissue homogenates and quantified using ELISA assay. (D) Relative tumor/normal ratio value of Alox5 and 5-HETE levels in individual gastric cancer patients. Alox5 or 5-HETE level in normal tissues were set as 1 (indicated by a red line)