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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-38050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALOX5 Recombinant Rabbit Monoclonal Antibody (ARC1926)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: Raji, Rat lung
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- ARC1926
- Vial size
- 100 µL
- Concentration
- 1.45 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Short-term high fat feeding induces inflammatory responses of tuft cells and mucosal barrier cells in the murine stomach.
Widmayer P, Pregitzer P, Breer H
Histology and histopathology 2023 Mar;38(3):273-286
Histology and histopathology 2023 Mar;38(3):273-286
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of Raji cells using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of Rat lung using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of ALOX5 in U-2 OS cells using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of ALOX5 in U-2 OS cells using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALOX5 in human appendix tissue using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALOX5 in mouse spleen tissue using ALOX5 Recombinant Monoclonal Antibody (Product # MA5-38050) at a dilution of 1:100.
