Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PPS001 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat/Primate Phospho-5-Lipoxygenase (S523) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human and rat 5-Lipoxygenase phosphorylated at S523 in Western blots.
- Reactivity
- Human, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Phospho-5-Lipoxygenase (S523) by Western Blot. Western blot of rat brain (cortex) tissue lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at S523 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-5-Lipoxygenase (S523). The immunolabeling is completely eliminated by treatment with lambda-PPase.
