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LearnMAB457-100
antibody from R&D Systems
Targeting: CCL21
6Ckine, CKb9, ECL, exodus-2, SCYA21, SLC, TCA4
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB457-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL21/6Ckine Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 6Ckine, rhMIP-1 alpha , rhMIP-1 beta , recombinant mouse (rm) MIP-1 alpha , rmMIP-1 beta , rhMIP-1 delta , rmMIP-1 gamma , rmMIP-2, rhMIP-3 alpha , rmMIP-3 alpha , rhMIP-3 beta , rmMIP-3 beta , rhTeck, or rmTeck.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P84444- Isotype
- IgG
- Antibody clone number
- 59106
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Thymic T cell development and progenitor localization depend on CCR7.
Herpes simplex virus (HSV) amplicon-mediated codelivery of secondary lymphoid tissue chemokine and CD40L results in augmented antitumor activity.
Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
Misslitz A, Pabst O, Hintzen G, Ohl L, Kremmer E, Petrie HT, Förster R
The Journal of experimental medicine 2004 Aug 16;200(4):481-91
The Journal of experimental medicine 2004 Aug 16;200(4):481-91
Herpes simplex virus (HSV) amplicon-mediated codelivery of secondary lymphoid tissue chemokine and CD40L results in augmented antitumor activity.
Tolba KA, Bowers WJ, Muller J, Housekneckt V, Giuliano RE, Federoff HJ, Rosenblatt JD
Cancer research 2002 Nov 15;62(22):6545-51
Cancer research 2002 Nov 15;62(22):6545-51
Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
Ato M, Stäger S, Engwerda CR, Kaye PM
Nature immunology 2002 Dec;3(12):1185-91
Nature immunology 2002 Dec;3(12):1185-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL21/6Ckine in Mouse Thymus. CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
