MA5-34721

antibody from Invitrogen Antibodies

Targeting: ACTR3 ARP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34721

Provider

Invitrogen Antibodies

Product name

Arp3 Recombinant Rabbit Monoclonal Antibody (JB33-44)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A431, mouse placenta tissue lysate, mouse thymus tissue lysate, HUVEC, LOVO, SiHa, rat kidney tissue, human placenta tissue, human stomach cancer tissue, mouse kidney tissue, HL-60.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JB33-44

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Arp3 in Lane 1: A431, Lane 2: Mouse placenta, Lane 3: Mouse thymus. Samples were incubated with Arp3 monoclonal antibody (Product # MA5-34721), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Arp3 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Arp3 monoclonal antibody (Product # MA5-34721), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Arp3 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Arp3 monoclonal antibody (Product # MA5-34721), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Arp3 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Arp3 monoclonal antibody (Product # MA5-34721), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Arp3 in paraffin-embedded rat kidney tissue. Samples were incubated with Arp3 monoclonal antibody (Product # MA5-34721), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Arp3 in paraffin-embedded mouse kidney tissue. Samples were incubated with Arp3 monoclonal antibody (Product # MA5-34721), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Arp3 in paraffin-embedded human stomach cancer tissue. Samples were incubated with Arp3 monoclonal antibody (Product # MA5-34721), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Arp3 in paraffin-embedded human placenta tissue. Samples were incubated with Arp3 monoclonal antibody (Product # MA5-34721), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Arp3 in HL-60 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Arp3 monoclonal antibody (Product # MA5-34721) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.