PA5-17288

antibody from Invitrogen Antibodies

Targeting: ACTR3 ARP3

Antibody data

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Validation data

Product number: PA5-17288 - Provider product page
Provider: Invitrogen Antibodies
Product name: Arp3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: It is not recommended to aliquot this antibody. This antibody is not cross-reactive with endogenous levels of total ARP2 protein.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 66 µg/mL
Storage: -20°C

ACTR3 protein structure - PA5-17288 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ARP3 in extracts from Jurkat and COS cells using ARP3 polyclonal antibody (Product # PA5-17288).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Arp3 was achieved by transfecting MCF-7 cells with Arp3 specific siRNAs (Silencer® select Product # s19640, s19641). Western blot analysis (Fig. a) was performed using whole cell extracts from the MCF-7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Arp3 Rabbit Polyclonal Antibody (Product # PA5-17288, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Arp3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Arp3 Polyclonal Antibody (Product # PA5-17288) and an ~47 kDa band was observed across the panel tested except Mouse and Rat Skeletal Muscle. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF-7 (Lane 2), PC-3 (Lane 3), U-87 MG (Lane 4), HeLa (Lane 5), HEL 92.1.7 (Lane 6), tissue extracts (30 µg lysate) of Mouse Thymus (Lane 7), Mouse Skeletal Muscle (Lane 8), Rat Thymus (Lane 9) and Rat Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).