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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-13156 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-13156, RRID:AB_627385
- Product name
- Anti-CYCS
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Cadmium induces intracellular Ca2+- and H2O2-dependent apoptosis through JNK- and p53-mediated pathways in skin epidermal cell line.
Bicyclol: a novel antihepatitis drug with hepatic heat shock protein 27/70-inducing activity and cytoprotective effects in mice.
A novel histone deacetylase 8 (HDAC8)-specific inhibitor PCI-34051 induces apoptosis in T-cell lymphomas
Conjugated linoleic acid induces apoptosis of murine mammary tumor cells via Bcl-2 loss.
The major apoptotic pathway activated and suppressed by poliovirus.
Son YO, Lee JC, Hitron JA, Pan J, Zhang Z, Shi X
Toxicological sciences : an official journal of the Society of Toxicology 2010 Jan;113(1):127-37
Toxicological sciences : an official journal of the Society of Toxicology 2010 Jan;113(1):127-37
Bicyclol: a novel antihepatitis drug with hepatic heat shock protein 27/70-inducing activity and cytoprotective effects in mice.
Bao XQ, Liu GT
Cell stress & chaperones 2008 Sep;13(3):347-55
Cell stress & chaperones 2008 Sep;13(3):347-55
A novel histone deacetylase 8 (HDAC8)-specific inhibitor PCI-34051 induces apoptosis in T-cell lymphomas
S Balasubramanian, J Ramos, W Luo, M Sirisawad, E Verner, J J Buggy
Leukemia 2008 Feb;22(5):1026-1034
Leukemia 2008 Feb;22(5):1026-1034
Conjugated linoleic acid induces apoptosis of murine mammary tumor cells via Bcl-2 loss.
Ou L, Ip C, Lisafeld B, Ip MM
Biochemical and biophysical research communications 2007 May 18;356(4):1044-9
Biochemical and biophysical research communications 2007 May 18;356(4):1044-9
The major apoptotic pathway activated and suppressed by poliovirus.
Belov GA, Romanova LI, Tolskaya EA, Kolesnikova MS, Lazebnik YA, Agol VI
Journal of virology 2003 Jan;77(1):45-56
Journal of virology 2003 Jan;77(1):45-56
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 12
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
