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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AF3922a - Provider product page
- Provider
- Abgent
- Product name
- "ERK3 / MAPK6 Antibody (internal region, near C-Term)"
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide-affinity purified goat antibody
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1mg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -1506°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with AF3922a (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "AF3922a (1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel)."
