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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- EB11926 - Provider product page
- Provider
- Everest Biotech
- Product name
- Goat anti-ERK3 / MAPK6 Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-ERK3 / MAPK6 Antibody
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
C-HSPVGSPLKSIQ- Epitope
- internal region (near C Terminus)
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with EB11926 (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB11926 (1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).
