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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB10662 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10662, RRID:AB_11189072
- Product name
- GAPDH monoclonal antibody, clone 1A10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant GAPDH.
- Isotype
- IgG
- Antibody clone number
- 1A10
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) against HeLa (1) , A-549 (2) , A-431 (3) , MCF-7 (4) , K-562 (5) , Jurkat (6) , HL-60 (7) , SKN-SH (8) and SK-BR-3 (9) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of methanol-fixed HepG2 (upper) and HeLa (bottom) cells using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma (upper) and kidney carcinoma (bottom) , showing cytoplasmic localization using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
