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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-47724 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-47724, RRID:AB_627678
- Product name
- Anti-GAPDH
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells.
Bioassays to monitor taspase1 function for the identification of pharmacogenetic inhibitors.
During hormone depletion or tamoxifen treatment of breast cancer cells the estrogen receptor apoprotein supports cell cycling through the retinoic acid receptor α1 apoprotein.
Organometallic iron(III)-salophene exerts cytotoxic properties in neuroblastoma cells via MAPK activation and ROS generation.
Patterns of mRNA and protein expression during minus-lens compensation and recovery in tree shrew sclera.
Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors.
Overexpression of ezrin inactivates NF2 tumor suppressor in glioblastoma.
The p53 core domain is a molten globule at low pH: functional implications of a partially unfolded structure.
Pin1 facilitates the phosphorylation-dependent ubiquitination of SF-1 to regulate gonadotropin beta-subunit gene transcription.
Lipophilic aroylhydrazone chelator HNTMB and its multiple effects on ovarian cancer cells.
Rhabdastrellic acid-A induced autophagy-associated cell death through blocking Akt pathway in human cancer cells.
Characterization of the plasma membrane localization and orientation of HPV16 E5 for cell-cell fusion.
White Tea extract induces lipolytic activity and inhibits adipogenesis in human subcutaneous (pre)-adipocytes.
Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation.
Mutual inhibition between Kaposi's sarcoma-associated herpesvirus and Epstein-Barr virus lytic replication initiators in dually-infected primary effusion lymphoma.
The pivotal role of c-Jun NH2-terminal kinase-mediated Beclin 1 expression during anticancer agents-induced autophagy in cancer cells
Gopalakrishna-Pillai S, Iverson LE
PloS one 2011;6(6):e20663
PloS one 2011;6(6):e20663
Bioassays to monitor taspase1 function for the identification of pharmacogenetic inhibitors.
Knauer SK, Fetz V, Rabenstein J, Friedl S, Hofmann B, Sabiani S, Schröder E, Kunst L, Proschak E, Thines E, Kindler T, Schneider G, Marschalek R, Stauber RH, Bier C
PloS one 2011;6(5):e18253
PloS one 2011;6(5):e18253
During hormone depletion or tamoxifen treatment of breast cancer cells the estrogen receptor apoprotein supports cell cycling through the retinoic acid receptor α1 apoprotein.
Salazar MD, Ratnam M, Patki M, Kisovic I, Trumbly R, Iman M, Ratnam M
Breast cancer research : BCR 2011 Feb 7;13(1):R18
Breast cancer research : BCR 2011 Feb 7;13(1):R18
Organometallic iron(III)-salophene exerts cytotoxic properties in neuroblastoma cells via MAPK activation and ROS generation.
Kim KK, Singh RK, Strongin RM, Moore RG, Brard L, Lange TS
PloS one 2011 Apr 29;6(4):e19049
PloS one 2011 Apr 29;6(4):e19049
Patterns of mRNA and protein expression during minus-lens compensation and recovery in tree shrew sclera.
Gao H, Frost MR, Siegwart JT Jr, Norton TT
Molecular vision 2011 Apr 12;17:903-19
Molecular vision 2011 Apr 12;17:903-19
Human parvovirus B19 causes cell cycle arrest of human erythroid progenitors via deregulation of the E2F family of transcription factors.
Wan Z, Zhi N, Wong S, Keyvanfar K, Liu D, Raghavachari N, Munson PJ, Su S, Malide D, Kajigaya S, Young NS
The Journal of clinical investigation 2010 Oct 1;120(10):3530-44
The Journal of clinical investigation 2010 Oct 1;120(10):3530-44
Overexpression of ezrin inactivates NF2 tumor suppressor in glioblastoma.
Morales FC, Molina JR, Hayashi Y, Georgescu MM
Neuro-oncology 2010 Jun;12(6):528-39
Neuro-oncology 2010 Jun;12(6):528-39
The p53 core domain is a molten globule at low pH: functional implications of a partially unfolded structure.
Bom AP, Freitas MS, Moreira FS, Ferraz D, Sanches D, Gomes AM, Valente AP, Cordeiro Y, Silva JL
The Journal of biological chemistry 2010 Jan 22;285(4):2857-66
The Journal of biological chemistry 2010 Jan 22;285(4):2857-66
Pin1 facilitates the phosphorylation-dependent ubiquitination of SF-1 to regulate gonadotropin beta-subunit gene transcription.
Luo Z, Wijeweera A, Oh Y, Liou YC, Melamed P
Molecular and cellular biology 2010 Feb;30(3):745-63
Molecular and cellular biology 2010 Feb;30(3):745-63
Lipophilic aroylhydrazone chelator HNTMB and its multiple effects on ovarian cancer cells.
Kim KK, Lange TS, Singh RK, Brard L
BMC cancer 2010 Feb 25;10:72
BMC cancer 2010 Feb 25;10:72
Rhabdastrellic acid-A induced autophagy-associated cell death through blocking Akt pathway in human cancer cells.
Li DD, Guo JF, Huang JJ, Wang LL, Deng R, Liu JN, Feng GK, Xiao DJ, Deng SZ, Zhang XS, Zhu XF
PloS one 2010 Aug 17;5(8):e12176
PloS one 2010 Aug 17;5(8):e12176
Characterization of the plasma membrane localization and orientation of HPV16 E5 for cell-cell fusion.
Hu L, Ceresa BP
Virology 2009 Oct 10;393(1):135-43
Virology 2009 Oct 10;393(1):135-43
White Tea extract induces lipolytic activity and inhibits adipogenesis in human subcutaneous (pre)-adipocytes.
Söhle J, Knott A, Holtzmann U, Siegner R, Grönniger E, Schepky A, Gallinat S, Wenck H, Stäb F, Winnefeld M
Nutrition & metabolism 2009 May 1;6:20
Nutrition & metabolism 2009 May 1;6:20
Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation.
Oser M, Yamaguchi H, Mader CC, Bravo-Cordero JJ, Arias M, Chen X, Desmarais V, van Rheenen J, Koleske AJ, Condeelis J
The Journal of cell biology 2009 Aug 24;186(4):571-87
The Journal of cell biology 2009 Aug 24;186(4):571-87
Mutual inhibition between Kaposi's sarcoma-associated herpesvirus and Epstein-Barr virus lytic replication initiators in dually-infected primary effusion lymphoma.
Jiang Y, Xu D, Zhao Y, Zhang L
PloS one 2008 Feb 6;3(2):e1569
PloS one 2008 Feb 6;3(2):e1569
The pivotal role of c-Jun NH2-terminal kinase-mediated Beclin 1 expression during anticancer agents-induced autophagy in cancer cells
D-D Li, L-L Wang, R Deng, J Tang, Y Shen, J-F Guo, Y Wang, L-P Xia, G-K Feng, Q Q Liu, W-L Huang, Y-X Zeng, X-F Zhu
Oncogene 2008 Dec;28(6):886-898
Oncogene 2008 Dec;28(6):886-898
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human prostate shows moderate cytoplasmic and nuclear positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
