Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- OAGA00012 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- GAPDH antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- GAPDH antibody detects GAPDH protein by Western blot analysis.A. 30 ug 293T whole cell lysate/extractB. 30 ug NIH-3T3 whole cell lysate/extractC. 30 ug mouse brain lysate/extractD. 30 ug PC-12 whole cell lysate/extractE. 30 ug rat brain lysate/extract10 % SDS-PAGEGAPDH antibody (OAGA00012) dilution: 1:10000
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH(OAGA00012) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(red) at 1:2000.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Salivary gland cancer, using GAPDH(OAGA00012) antibody at 1:500 dilution.