Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C210926 - Provider product page
- Provider
- LSBio
- Product name
- GAPDH Antibody LS-C210926
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using Glyceraldehyde-3-phosphate dehydrogenase antibody at 2.6 ug/ml. Lane 1: HepG2 whole cell lysate, Lane 2: Mouse brain lysate, Lane 3: Mouse skeletal muscle lysate
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, 293T cell lysate; Lane 3, Jurkat cell lysates; Lane 4, Mouse kidney extract; Lane 5, Rat muscle extract; Lane 6, Rat lung extract; and Lane 7, Zebrafish extract.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using GADPH antibody at 2 ug/ml. Lane 1, HeLa cell lysate; Lane 2, A549 cell lysate; Lane 3, HepG2 cell lysates; Lane 4, K562 cell lysate; Lane 5, Jurkat cell lysate; Lane 6, MDA-MB-231 cell lysate; Lane 7, LO2 cell lysate; Lane 8, U251 cell lysate; and Lane 9, MCF-7 cell lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate All lanes: GAPDH antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 32 kDa Observed band size: 37 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry with GADPH antibody at a 1:50 dilution on paraffin-embdeed mouse liver