Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C291113 - Provider product page
- Provider
- LSBio
- Product name
- GAPDH Antibody (aa150-200) LS-C291113
- Antibody type
- Monoclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine, Sheep, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Storage
- Store at 2°C to 8°C for up to 1 year.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human GAPDH by Western Blot. Samples: Whole Cell Lysate from HeLa cells (50, 15 and 5 ug) 293T (T; 50 ug) and NIH3T3 (M; 50 ug). Antibodies: Purified mouse monoclonal anti-GAPDH antibody was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human and mouse GAPDH by WB of HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Mouse anti-GAPDH antibody [12G11]. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human GAPDH by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: monoclonal anti-GAPDH antibody used at a dilution of 1:200.