AM1020a

antibody from Abgent

Targeting: GAPDH GAPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1020a

Provider

Abgent

Proper citation

Abgent Cat#AM1020a, RRID:AB_1278045

Product name

GAPDH Antibody (Ascites)

Antibody type

Monoclonal

Antigen

Other

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Vial size

0.1 ml

Concentration

None

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020a) in HepG2,A375,A2058,NCI-H460 and CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the Mab ascites (1:25000 dilution)."

Primary Ab dilution

1:25000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of GAPDH Antibody (Ascites) (Cat#AM1020a) with hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH Monoclonal Antibody (Cat.#AM1020a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:1000~10000