Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM1020a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1020a, RRID:AB_1278045
- Product name
- GAPDH Antibody (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Vial size
- 0.1 ml
- Concentration
- None
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020a) in HepG2,A375,A2058,NCI-H460 and CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the Mab ascites (1:25000 dilution)."
- Primary Ab dilution
- 1:25000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GAPDH Antibody (Ascites) (Cat#AM1020a) with hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH Monoclonal Antibody (Cat.#AM1020a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:1000~10000