Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM1020b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1020b, RRID:AB_2107462
- Product name
- GAPDH Antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Vial size
- 400 µl
- Concentration
- 0.44 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020b) in CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the purified Mab.
- Primary Ab dilution
- 1:2000~10000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
- Primary Ab dilution
- 1:25
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining."
- Primary Ab dilution
- 1:25
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining."
- Primary Ab dilution
- 1:25