AM1020b

antibody from Abgent

Targeting: GAPDH GAPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1020b

Provider

Abgent

Proper citation

Abgent Cat#AM1020b, RRID:AB_2107462

Product name

GAPDH Antibody

Antibody type

Monoclonal

Antigen

Other

Description

Purified Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Vial size

400 µl

Concentration

0.44 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020b) in CEM cell line lysates (35ug/lane). GAPDH(arrow) was detected using the purified Mab.

Primary Ab dilution

1:2000~10000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Primary Ab dilution

1:25

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining."

Primary Ab dilution

1:25

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining."

Primary Ab dilution

1:25