NBP2-37828

antibody from Novus Biologicals

Targeting: GAPDH GAPD

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-37828

Provider

Novus Biologicals

Product name

Mouse Monoclonal GAPDH Antibody

Antibody type

Monoclonal

Description

Protein A purified. This GAPDH antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples. This product has been successfully used in Western blot, ELISA, and immunofluorescence.

Reactivity

Human, Mouse, Rat, Bacteria, Chicken/Avian, Hamster, Porcine, Rabbit, Yeast

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Myocardin and myocardin-related transcription factor-A synergistically mediate actin cytoskeletal-dependent inhibition of liver fibrogenesis.

Shi Z, Ren M, Rockey DC
American journal of physiology. Gastrointestinal and liver physiology 2020 Mar 1;318(3):G504-G517

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Theoretical molecular weight: 36 kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. GAPDH theoretical molecular weight: 36 kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of human kidney tissue (right) compared with a negative control without primary antibody (left).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of mouse colon tissue (right) compared with a negative control without primary antibody (left).

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in Hela cells (green) compared to an isotype control (blue).