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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- TA302944 - Provider product page
- Provider
- OriGene
- Product name
- Goat polyclonal anti-GAPDH antibody(C-terminal), Loading control
- Antibody type
- Polyclonal
- Description
- Goat polyclonal anti-GAPDH antibody(C-terminal), Loading control
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- GAPDH
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- NULL
Submitted references Pseudosyndactyly - an inflammatory and fibrotic wound healing disorder in recessive dystrophic epidermolysis bullosa.
Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways.
Breitenbach J, Gruber C, Klausegger A, Trost A, Bogner B, Reitsamer H, Bauer JW
Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG 2015 Dec;13(12):1257-66
Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG 2015 Dec;13(12):1257-66
Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways.
Herzog J, Rid R, Wagner M, Hundsberger H, Eger A, Bauer J, Önder K
BMC research notes 2015 Dec 15;8:785
BMC research notes 2015 Dec 15;8:785
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302944 (0.1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated GAPDH knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302944 staining (0.01ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TA302944 (0.1ug/ml) staining of PFA-fixed HeLa before (top) and after (bottom) si-RNA-mediated GAPDH knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain (right).
- Validation comment
- IF
