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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA308884 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal antibody to GAPDH (glyceraldehyde-3-phosphate dehydrogenase)
- Reactivity
- Canine, Porcine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GAPDH
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Trastuzumab increases the sensitivity of HER2-amplified human gastric cancer cells to oxaliplatin and cisplatin by affecting the expression of telomere-associated proteins.
Liu Y, Ling Y, Qi Q, Zhu M, Wan M, Zhang Y, Zhang C
Oncology letters 2015 Feb;9(2):999-1005
Oncology letters 2015 Feb;9(2):999-1005
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody detects GAPDH protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug NIH-3T3 whole cell lysate/extract. C. 30 ug mouse brain lysate/extract. D. 30 ug PC-12 whole cell lysate/extract. E. 30 ug rat brain lysate/ex
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody detects GAPDH protein by Western blot analysis. A. 0.1 ug 293T whole cell lysate/extract. B. 1 ug 293T whole cell lysate/extract. C. 10 ug 293T whole cell lysate/extract. 12 % SDS-PAGE. GAPDH antibody (TA308884) dilution: 1:10000
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract |. D. 30 ug HepG2 whole cell lysate/extract. 10 % SDS-PAGE. GAPDH anti
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody detects GAPDH protein by western blot analysis. A. 20 ug 293T whole cell lysate/extract. B. 10 ug 293T whole cell lysate/extract. C. 5 ug 293T whole cell lysate/extract. D. 1 ug 293T whole cell lysate/extract. 10 % SDS-PAGE. GAPDH antibody
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- GAPDH antibody detects GAPDH protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 10 % SDS-PAGE. GAPDH antibody (TA308884) dilution: 1:10000
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western blot analysis of GAPDH protein expression by using anti-GAPDH antibody in NCI-N87 cells. Dilution: 1:10000
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH(TA308884) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (red) at 1:2000.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Salivary gland cancer, using GAPDH(TA308884) antibody at 1:500 dilution.
- Validation comment
- IHC
