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- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- TA337137 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal GAPDH/G3PDH Antibody (13H12)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal GAPDH/G3PDH Antibody (13H12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GAPDH
- Antibody clone number
- 13H12
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
Submitted references Effects of 2-(2-Chlorophenyl)ethylbiguanide on ERAD Component Expression in HT-29 Cells Under a Serum- and Glucose-Deprived Condition.
Oh-Hashi K, Matsumoto S, Sakai T, Hirata Y, Okuda K, Nagasawa H
Applied biochemistry and biotechnology 2019 Aug;188(4):1009-1021
Applied biochemistry and biotechnology 2019 Aug;188(4):1009-1021
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [TA337137] - analysis of GAPDH in HeLa and HEK 293 cells (25ug/lane) using anti-GAPDH antibody. Image from verified customer review.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [TA337137] - WB detection of GAPDH protein in HeLa cells lysate using GAPDH antibody (clone 13H12) in (A) the absence and (B) the presence of immunizing peptide.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [TA337137] - WB detection of GAPDH protein in lysates of (A) HEK 293 cells and (B) Drosophila larvae using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody (13H12) [TA337137] - WB detection of GAPDH protein in lysates of Mouse cell lines (A) NIH 3T3 (B) RAW 264.7 using GAPDH antibody (clone 13H12) at a concentration of 0.25 ug/ml.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GAPDH Antibody (13H12) [TA337137] - GAPDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A dilution of 1:10 was used.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [TA337137] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human colon tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [TA337137] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of human rectal carcinoma tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [TA337137] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human breast tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [TA337137] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded section of normal human stomach tissue using GAPDH antibody (clone 13H12) at 5 ug/ml concentration.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody (13H12) [TA337137] - IHC-P detection GAPDH protein in a formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using GAPDH antibody (clone 13H12) at 5 ug/ml concentra
- Validation comment
- IHC
