GTX89740

antibody from GeneTex

Targeting: GAPDH GAPD

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX89740

Provider

GeneTex

Proper citation

GeneTex Cat#GTX89740, RRID:AB_10721581

Product name

GAPDH antibody, C-term

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Drosophila

Host

Goat

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB analysis of HeLa lysate control and after si-RNA-mediated GAPDH knock-down (35£gg per lane) using GAPDH antibody at a concentration of 0.1 £gg/ml. Level of knock-down relative to Actin expression level was determined by RT-PCR.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod no

GTX89740

KD Reagent Provider

Customized siRNA

KD Reagent Prod page

http://www.genetex.com

Antibody Lot Number

39224

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX89740 staining (0.01?g/ml) of 293 lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of HeLa cells before (top) and after (bottom) si-RNA-mediated GAPDH knock-down using GAPDH antibody at a concentration of 0.1 ug/ml (green). Nuclei were stained wuth DAPI stain (blue).