Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB8701 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8701, RRID:AB_10716171
- Product name
- GAPDH monoclonal antibody, clone AT8G4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant GAPDH.
- Isotype
- IgG
- Antibody clone number
- AT8G4
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases.
Alteration of intracellular structure and function of glyceraldehyde-3-phosphate dehydrogenase: a common phenotype of neurodegenerative disorders?
Chuang DM, Hough C, Senatorov VV
Annual review of pharmacology and toxicology 2005;45:269-90
Annual review of pharmacology and toxicology 2005;45:269-90
Alteration of intracellular structure and function of glyceraldehyde-3-phosphate dehydrogenase: a common phenotype of neurodegenerative disorders?
Mazzola JL, Sirover MA
Neurotoxicology 2002 Oct;23(4-5):603-9
Neurotoxicology 2002 Oct;23(4-5):603-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with GAPDH monoclonal antibody, clone AT8G4 (Cat # MAB8701) (1 : 6000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and GAPDH monoclonal antibody, clone AT8G4 (Cat # MAB8701) (1 : 500) with Texas Red (Red).
- Validation comment
- Immunofluorescence