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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NB300-324 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-324, RRID:AB_10002458
- Product name
- Rabbit Polyclonal GAPDH Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The miR-15/107 Family of microRNA Genes Regulates CDK5R1/p35 with Implications for Alzheimer's Disease Pathogenesis.
Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap.
Moncini S, Lunghi M, Valmadre A, Grasso M, Del Vescovo V, Riva P, Denti MA, Venturin M
Molecular neurobiology 2017 Aug;54(6):4329-4342
Molecular neurobiology 2017 Aug;54(6):4329-4342
Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap.
Jiang H, Hurt KJ, Breen K, Stabler SP, Allen RH, Orlicky DJ, Maclean KN
Biology open 2015 Aug 14;4(9):1154-62
Biology open 2015 Aug 14;4(9):1154-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH, theoretical molecular weight 36 kDa. Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: GAPDH Antibody [NB300-324] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded mouse squamous cell carcinoma tissue sections with NB300-324 at a dilution of 1:200.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded human lung carcinoma tissue sections with NB300-324 at a dilution of 1:200
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GAPDH Antibody [NB300-324] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB100-79808). Detection: DAB
