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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB2462 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2462, RRID:AB_1575741
- Product name
- HSPA5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant HSPA5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Structure and expression of the three MHC-linked HSP70 genes.
Human major histocompatibility complex contains genes for the major heat shock protein HSP70.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA, Mammalian Gene Collection Program Team
Proceedings of the National Academy of Sciences of the United States of America 2002 Dec 24;99(26):16899-903
Proceedings of the National Academy of Sciences of the United States of America 2002 Dec 24;99(26):16899-903
Structure and expression of the three MHC-linked HSP70 genes.
Milner CM, Campbell RD
Immunogenetics 1990;32(4):242-51
Immunogenetics 1990;32(4):242-51
Human major histocompatibility complex contains genes for the major heat shock protein HSP70.
Sargent CA, Dunham I, Trowsdale J, Campbell RD
Proceedings of the National Academy of Sciences of the United States of America 1989 Mar;86(6):1968-72
Proceedings of the National Academy of Sciences of the United States of America 1989 Mar;86(6):1968-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The HSPA5 polyclonal antibody (Cat # PAB2462) is used in Western blot to detect HSPA5 in mouse liver tissue lysate (lane 1) and HL-60 cell lysate (lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HSPA5 polyclonal antibody (Cat # PAB2462) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with HSPA5 polyclonal antibody (Cat # PAB2462) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
