NB110-61640

antibody from Novus Biologicals

Targeting: HSP90AB1 HSPC2, HSPCB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-61640

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-61640, RRID:AB_963937

Product name

Mouse Monoclonal HSP90 beta Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects 90 kDa protein. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Rabbit

Host

Mouse

Isotype

IgG

Vial size

0.2 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. 15 ug protein each lane. Block: 1.5% BSA for 30 minutes at RT. Primary antibody at 1:1000 for 2 hours at RT. Secondary antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - Human HeLa cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP90AB1 Antibody (H90-10) [NB110-61640] - HCC827 human non-small cell lung cancer cell line and A549 cells lysates. Image from verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: HSP90AB1 Antibody (H90-10) [NB110-61640] - Mouse inflammed colon tissue. Fixation: Formalin. Primary antibody at 1:10000 for 12 hours at 4C. Secondary antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HSP90AB1 Antibody (H90-10) [NB110-61640] - Cancerous human colon tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HSP90AB1 Antibody (H90-10) [NB110-61640] - Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: HSP90AB1 Antibody (H90-10) [NB110-61640] - An intracellular stain was performed on Jurkat cells with antibody NB110-61640APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. An antibody dilution of 1:100 was used and cells were incubated for 30 minutes at room temperature. Image using the Allophycocyanin form of this antibody.