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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00004204-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004204-M01, RRID:AB_540384
- Product name
- Mouse Monoclonal MeCP2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MECP2 - methyl CpG binding protein 2 (Rett syndrome)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Rett Syndrome Mutant Neural Cells Lacks MeCP2 Immunoreactive Bands.
MeCP2 SUMOylation rescues Mecp2-mutant-induced behavioural deficits in a mouse model of Rett syndrome.
Bueno C, Tabares-Seisdedos R, Moraleda JM, Martinez S
PloS one 2016;11(4):e0153262
PloS one 2016;11(4):e0153262
MeCP2 SUMOylation rescues Mecp2-mutant-induced behavioural deficits in a mouse model of Rett syndrome.
Tai DJ, Liu YC, Hsu WL, Ma YL, Cheng SJ, Liu SY, Lee EH
Nature communications 2016 Feb 4;7:10552
Nature communications 2016 Feb 4;7:10552
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in NIH/3T3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6 Analysis of MECP2 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: MeCP2 Antibody (4B6) [H00004204-M01] - Detection limit for recombinant GST tagged MECP2 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
