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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AB0063-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0063-200, RRID:AB_2333160
- Product name
- Anti-VEGFA
- Antibody type
- Polyclonal
- Antigen
- Purified recombinant human VEGFA isoform 6 produced in E. coli.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Zebrafish
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 200 µl
- Concentration
- 3 mg/ml
- Storage
- For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance.
Rodrigues T, Matafome P, Sereno J, Almeida J, Castelhano J, Gamas L, Neves C, Gonçalves S, Carvalho C, Arslanagic A, Wilcken E, Fonseca R, Simões I, Conde SV, Castelo-Branco M, Seiça R
Scientific reports 2017 May 10;7(1):1698
Scientific reports 2017 May 10;7(1):1698
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- 50 ng of recombinant protein per lane
- Sample type
- MBP-VEGFA iso6
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cells were fixed with methanol
- Sample type
- Primary RPE cells
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated
