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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- ELISA [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- PAB14126 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14126, RRID:AB_10552242
- Product name
- MBD1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MBD1.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references hMLH1 promoter methylation and silencing in primary endometrial cancers are associated with specific alterations in MBDs occupancy and histone modifications.
Xiong Y, Dowdy SC, Eberhardt NL, Podratz KC, Jiang SW
Gynecologic oncology 2006 Oct;103(1):321-8
Gynecologic oncology 2006 Oct;103(1):321-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of MBD1 polyclonal antibody (Cat # PAB14126), crude serum and Flow Through in antigen coated wells.By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 16,500.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ChIP assays were performed using the U-2 OS (human osteosarcoma cell line), MBD1 polyclonal antibody (Cat # PAB14126) and optimized PCR primer sets.Chromatin sheared from 1x106 cells and 1.7 ug of MBD1 antibody or beads only were used per ChIP experiment.Figure shows the recovery as a % of the input DNA.Upper : Recovery by MBD1 or beads only of the MLH1 promoter, which specifically binds MBD1 (ref 1).Bottom : Recovery of the CDC6 promoter (used as a negative control) by MBD1 or beads only.
- Validation comment
- ChIP
