H00009618-D01P

antibody from Abnova Corporation

Targeting: TRAF4 CART1, MLN62, RNF83

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009618-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009618-D01P, RRID:AB_1581471

Product name

TRAF4 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human TRAF4 protein.

Antigen sequence

MPGFDYKFLEKPKRRLLCPLCGKPMREPVQVSTCG
HRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDP
ELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNT
CSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLK
CEFCGCDFSGEAYESHEGMCPQESVYCENKCGARM
MRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSH
QYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNT
ALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAM
MCALVSRQRQELQELRRELEELSVGSDGVLIWKIG
SYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSA
FLNGNGSGEGTHLSLYIRVLPGAFDNLLEWPFARR
VTFSLLDQSDPGLAKPQHVTETFHPDPNWKNFQKP
GTWRGSLDESSLGFGYPKFISHQDIRKRNYVRDDA
VFIRAAVELPRKILS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TRAF4 expression in transfected 293T cell line (H00009618-T02) by TRAF4 MaxPab polyclonal antibody.Lane 1: TRAF4 transfected lysate(53.50 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to TRAF4 on HeLa cell. [antibody concentration 20 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between TRAF4 and TRAF6. HeLa cells were stained with anti-TRAF4 rabbit purified polyclonal 1:1200 and anti-TRAF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)