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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 66755-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAF4 Monoclonal Antibody (2C9A11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: GHRFCDTCL QEFLSEGVFK CPEDQLPLDY AKIYPDPELE VQVLGLPIRC IHSEEGCRWS GPLRHLQGHL NTCSFNVIPC PNRCPMKLSR RDLPAHLQHD CPKRRLKCEF CGCDFSGEAY ESHEGMCPQE SVYCENKCGA RMMRRLLAQH ATSECPKRTQ PCTYCTKEFV FDTIQSHQYQ CPRLPVACPN QCGVGTVARE DLPGHLKDSC NTALVLCPFK DSGCKHRCPK LAMARHVEES VKPHLAMMCA LVSRQRQELQ ELRRELEELS VGSD (35-307 aa encoded by BC001769)
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2C9A11
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66755-1-IG (TRAF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 66755-1-IG (TRAF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66755-1-Ig (TRAF4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66755-1-IG (TRAF4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66755-1-IG (TRAF4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
