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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00008940-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008940-M04, RRID:AB_2205993
- Product name
- Mouse Monoclonal Topo III beta Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TOP3B - topoisomerase (DNA) III beta
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topo III beta Antibody (1H4) [H00008940-M04] - Analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M04), clone 1H4. Lane 1: TOP3B transfected lysatE (96.662 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Topo III beta Antibody (1H4) [H00008940-M04] - Detection limit for recombinant GST tagged TOP3B is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Topo III beta Antibody (1H4) [H00008940-M04] - Analysis of monoclonal antibody to TOP3B on HeLa cell. Antibody concentration 10 ug/ml
