R1024

antibody from Acris Antibodies GmbH

Targeting: NFYA CBF-B, HAP2, NF-YA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Immunoelectron microscopy (Recommended by provider)

Gel shift (Recommended by provider)

Antibody Data

Product number

R1024

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1024, RRID:AB_1005686

Product name

anti NFYA

Antibody type

Polyclonal

Antigen

NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.1 ml

Concentration

90.0 mg/ml (by Refractometry)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein were subjected to analysis by SDSPAGE and immunoblot. After transfer, the membrane was probed using anti-NF-YA as the primary antibody at a 1/1000 dilution, followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L]. Signal was developed using an ECL kit followed by autoradiography using a 30'' exposure. The antibody recognizes NF-YA as a doublet of ~ 42 kDa in size.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin Fixed Paraffin Embedded stained with NFYA Antibody Cat.-No R1024. Antigen retrieval: not required. Primary antibody: NF-Y antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1/10,000 for 45 min at RT. Localization: NF-Y is nuclear. Staining: NF-Y subunit A as precipitated red signal with hematoxylin purple nuclear counterstain.