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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF8064 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DAB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human DAB2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P98082- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human DAB2 by Western Blot. Western blot results for A172, HeLa and MCF 10A cell line lysates. A PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human DAB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8064) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DAB2 at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DAB2 in A172 Human Cell Line. DAB2 was detected in immersion fixed A172 cells using Goat Anti-Human DAB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8064) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized in the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DAB2 in Human Prostate. DAB2 was detected in formalin fixed paraffin-embedded sections of human prostate using Goat Anti-Human DAB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8064) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
