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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 66166-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TBP Monoclonal Antibody (2H3B2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).
- Antibody clone number
- 2H3B2
- Concentration
- 1.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-IG at a dilution of 1:8000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pig liver tissue were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 66166-1-IG (TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- I After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66166-1-IG ( TBP Antibody) at dilution of 1:650 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.20ug TBP antibody (66166-1-IG, red) and control antibody (blue). Fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TBP (IP:66166-1-IG, 3ug; Detection:66166-1-IG 1:800) with HEK-293 cells lysate 1800ug.
