Explore
Validate
LearnALX-804-370-C100
antibody from Enzo Life Sciences
Targeting: DNMT3A
Western blot
Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ALX-804-370-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-370-C100, RRID:AB_2051312
- Product name
- Dnmt3a (mouse) monoclonal antibody (64B1446)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 64B1446
- Vial size
- 100 μg
- Storage
- -20°C
Submitted references Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model.
Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system.
Myc represses transcription through recruitment of DNA methyltransferase corepressor.
De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine.
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer.
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin.
A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation.
Lsh, a member of the SNF2 family, is required for genome-wide methylation.
Stempak JM, Sohn KJ, Chiang EP, Shane B, Kim YI
Carcinogenesis 2005 May;26(5):981-90
Carcinogenesis 2005 May;26(5):981-90
Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system.
Feng J, Chang H, Li E, Fan G
Journal of neuroscience research 2005 Mar 15;79(6):734-46
Journal of neuroscience research 2005 Mar 15;79(6):734-46
Myc represses transcription through recruitment of DNA methyltransferase corepressor.
Brenner C, Deplus R, Didelot C, Loriot A, Viré E, De Smet C, Gutierrez A, Danovi D, Bernard D, Boon T, Pelicci PG, Amati B, Kouzarides T, de Launoit Y, Di Croce L, Fuks F
The EMBO journal 2005 Jan 26;24(2):336-46
The EMBO journal 2005 Jan 26;24(2):336-46
De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine.
Oka M, Meacham AM, Hamazaki T, Rodić N, Chang LJ, Terada N
Oncogene 2005 Apr 28;24(19):3091-9
Oncogene 2005 Apr 28;24(19):3091-9
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer.
Leu YW, Yan PS, Fan M, Jin VX, Liu JC, Curran EM, Welshons WV, Wei SH, Davuluri RV, Plass C, Nephew KP, Huang TH
Cancer research 2004 Nov 15;64(22):8184-92
Cancer research 2004 Nov 15;64(22):8184-92
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin.
Yan Q, Cho E, Lockett S, Muegge K
Molecular and cellular biology 2003 Dec;23(23):8416-28
Molecular and cellular biology 2003 Dec;23(23):8416-28
A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation.
Chen T, Ueda Y, Xie S, Li E
The Journal of biological chemistry 2002 Oct 11;277(41):38746-54
The Journal of biological chemistry 2002 Oct 11;277(41):38746-54
Lsh, a member of the SNF2 family, is required for genome-wide methylation.
Dennis K, Fan T, Geiman T, Yan Q, Muegge K
Genes & development 2001 Nov 15;15(22):2940-4
Genes & development 2001 Nov 15;15(22):2940-4
No comments: Submit comment
Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of Dnmt3a using ALX-804-370 at 2µg/ml against 10µg of 293 cell lysate transfected with either mouse Dnmt3a (lane A) or mouse Dnmt3b (lane B).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunostaining of Dnmt3a using ALX-804-370 at 5µg/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.
