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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [98]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [7]
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- Product number
- NB120-13888 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-13888, RRID:AB_2261802
- Product name
- Mouse Monoclonal DNMT3A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A genome-scale map of DNA methylation turnover identifies site-specific dependencies of DNMT and TET activity.
SOX3 promotes generation of committed spermatogonia in postnatal mouse testes.
Gain-of-function DNMT3A mutations cause microcephalic dwarfism and hypermethylation of Polycomb-regulated regions.
Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency.
Examining How the MAFB Transcription Factor Affects Islet β-Cell Function Postnatally.
Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells.
DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
Identification of dynamic undifferentiated cell states within the male germline.
MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer.
CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes.
Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming.
Intragenic DNA methylation prevents spurious transcription initiation.
Raptor regulates functional maturation of murine beta cells.
Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity.
Epigenetic determinants of space radiation-induced cognitive dysfunction.
RA-Induced Transcriptional Silencing of Checkpoint Kinase-2 through Promoter Methylation by Dnmt3b Is Required for Neuronal Differentiation of P19 Cells.
Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2.
Immunohistochemical expression of DNA methyltransferases 1, 3a, and 3b in actinic cheilitis and lip squamous cell carcinomas.
Inhibition of TGF-β Signaling Promotes Human Pancreatic β-Cell Replication.
Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway.
De novo DNA methylation drives 5hmC accumulation in mouse zygotes.
Mapping global changes in nuclear cytosine base modifications in the early mouse embryo.
Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells.
Maternal TET3 is dispensable for embryonic development but is required for neonatal growth.
Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1.
DNA methylation directs functional maturation of pancreatic β cells.
DNA methyltransferase immunohistochemical expression in odontogenic tumours.
Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics.
Deregulation of DNMT1, DNMT3B and miR-29s in Burkitt lymphoma suggests novel contribution for disease pathogenesis.
DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level.
Establishment of a conditional transgenic system using the 2A peptide in the female mouse germline.
Establishment of a conditional transgenic system using the 2A peptide in the female mouse germline.
Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase.
Blocking of JB6 cell transformation by tanshinone IIA: epigenetic reactivation of Nrf2 antioxidative stress pathway.
The combination of the prodrugs perforin-CEBPD and perforin-granzyme B efficiently enhances the activation of caspase signaling and kills prostate cancer.
A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype.
Apigenin reactivates Nrf2 anti-oxidative stress signaling in mouse skin epidermal JB6 P + cells through epigenetics modifications.
Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting.
Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia.
DNMT3B inhibits the re-expression of genes associated with induced pluripotency.
Epigenetic regulation of Dpp6 expression by Dnmt3b and its novel role in the inhibition of RA induced neuronal differentiation of P19 cells.
miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells.
Combined modulation of polycomb and trithorax genes rejuvenates β cell replication.
Analysis of DNA methylation change induced by Dnmt3b in mouse hepatocytes.
Naive pluripotency is associated with global DNA hypomethylation.
Hepatitis B virus inhibits liver regeneration via epigenetic regulation of urokinase-type plasminogen activator.
Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels.
Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1.
Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells.
Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases.
DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain.
Mothers matter too: benefits of temperature oviposition preferences in newts.
Clinical significance of the expression of DNA methyltransferase proteins in gastric cancer.
De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state.
Gene expression and mutation assessment provide clues of genetic and epigenetic mechanisms in liver tumors of oxazepam-exposed mice.
Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina.
DNA methyltransferase 3b preferentially associates with condensed chromatin.
Epigenetic reprogramming as a key contributor to melanocyte malignant transformation.
Epigenetic reprogramming as a key contributor to melanocyte malignant transformation.
Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.
DNA cytosine methylation in the bovine leukemia virus promoter is associated with latency in a lymphoma-derived B-cell line: potential involvement of direct inhibition of cAMP-responsive element (CRE)-binding protein/CRE modulator/activation transcription factor binding.
Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation.
A functional polymorphism in the DNA methyltransferase-3A promoter modifies the susceptibility in gastric cancer but not in esophageal carcinoma.
Immunohistochemical expression of DNA methyltransferases 1, 3a and 3b in oral leukoplakias and squamous cell carcinomas.
Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells.
Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres.
Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex.
Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation.
Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.
DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation.
IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencing.
Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein.
Coordinate regulation of DNA methyltransferase expression during oogenesis.
Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells.
Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation.
Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body.
Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome.
Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development.
Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b.
Expression of DNMT3A transcripts and nucleolar localization of DNMT3A protein in human testicular and fibroblast cells suggest a role for de novo DNA methylation in nucleolar inactivation.
DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma.
Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse.
Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells.
Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model.
Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system.
Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development.
Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development.
De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine.
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer.
Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation.
Expression of DNA methyltransferases in multistep hepatocarcinogenesis.
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin.
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin.
Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b.
Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b.
A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation.
Lsh, a member of the SNF2 family, is required for genome-wide methylation.
Ginno PA, Gaidatzis D, Feldmann A, Hoerner L, Imanci D, Burger L, Zilbermann F, Peters AHFM, Edenhofer F, Smallwood SA, Krebs AR, Schübeler D
Nature communications 2020 May 29;11(1):2680
Nature communications 2020 May 29;11(1):2680
SOX3 promotes generation of committed spermatogonia in postnatal mouse testes.
McAninch D, Mäkelä JA, La HM, Hughes JN, Lovell-Badge R, Hobbs RM, Thomas PQ
Scientific reports 2020 Apr 21;10(1):6751
Scientific reports 2020 Apr 21;10(1):6751
Gain-of-function DNMT3A mutations cause microcephalic dwarfism and hypermethylation of Polycomb-regulated regions.
Heyn P, Logan CV, Fluteau A, Challis RC, Auchynnikava T, Martin CA, Marsh JA, Taglini F, Kilanowski F, Parry DA, Cormier-Daire V, Fong CT, Gibson K, Hwa V, Ibáñez L, Robertson SP, Sebastiani G, Rappsilber J, Allshire RC, Reijns MAM, Dauber A, Sproul D, Jackson AP
Nature genetics 2019 Jan;51(1):96-105
Nature genetics 2019 Jan;51(1):96-105
Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency.
van Mierlo G, Dirks RAM, De Clerck L, Brinkman AB, Huth M, Kloet SL, Saksouk N, Kroeze LI, Willems S, Farlik M, Bock C, Jansen JH, Deforce D, Vermeulen M, Déjardin J, Dhaenens M, Marks H
Cell stem cell 2019 Jan 3;24(1):123-137.e8
Cell stem cell 2019 Jan 3;24(1):123-137.e8
Examining How the MAFB Transcription Factor Affects Islet β-Cell Function Postnatally.
Cyphert HA, Walker EM, Hang Y, Dhawan S, Haliyur R, Bonatakis L, Avrahami D, Brissova M, Kaestner KH, Bhushan A, Powers AC, Stein R
Diabetes 2019 Feb;68(2):337-348
Diabetes 2019 Feb;68(2):337-348
Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells.
Ravichandran M, Lei R, Tang Q, Zhao Y, Lee J, Ma L, Chrysanthou S, Lorton BM, Cvekl A, Shechter D, Zheng D, Dawlaty MM
Cell reports 2019 Aug 20;28(8):1993-2003.e5
Cell reports 2019 Aug 20;28(8):1993-2003.e5
DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
Chatterjee B, Lin MH, Chen CC, Peng KL, Wu MS, Tseng MC, Chen YJ, Shen CJ
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Nov;1861(11):1048-1061
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Nov;1861(11):1048-1061
Identification of dynamic undifferentiated cell states within the male germline.
La HM, Mäkelä JA, Chan AL, Rossello FJ, Nefzger CM, Legrand JMD, De Seram M, Polo JM, Hobbs RM
Nature communications 2018 Jul 19;9(1):2819
Nature communications 2018 Jul 19;9(1):2819
MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer.
Pang Y, Liu J, Li X, Xiao G, Wang H, Yang G, Li Y, Tang SC, Qin S, Du N, Zhang H, Liu D, Sun X, Ren H
Journal of cellular and molecular medicine 2018 Dec;22(12):6262-6274
Journal of cellular and molecular medicine 2018 Dec;22(12):6262-6274
CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes.
Xia L, Huang W, Bellani M, Seidman MM, Wu K, Fan D, Nie Y, Cai Y, Zhang YW, Yu LR, Li H, Zahnow CA, Xie W, Chiu Yen RW, Rassool FV, Baylin SB
Cancer cell 2017 May 8;31(5):653-668.e7
Cancer cell 2017 May 8;31(5):653-668.e7
Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming.
Mahadevan S, Sathappan V, Utama B, Lorenzo I, Kaskar K, Van den Veyver IB
Scientific reports 2017 Mar 20;7:44667
Scientific reports 2017 Mar 20;7:44667
Intragenic DNA methylation prevents spurious transcription initiation.
Neri F, Rapelli S, Krepelova A, Incarnato D, Parlato C, Basile G, Maldotti M, Anselmi F, Oliviero S
Nature 2017 Mar 2;543(7643):72-77
Nature 2017 Mar 2;543(7643):72-77
Raptor regulates functional maturation of murine beta cells.
Ni Q, Gu Y, Xie Y, Yin Q, Zhang H, Nie A, Li W, Wang Y, Ning G, Wang W, Wang Q
Nature communications 2017 Jun 9;8:15755
Nature communications 2017 Jun 9;8:15755
Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity.
Hoang TV, Horowitz ER, Chaffee BR, Qi P, Flake RE, Bruney DG, Rasor BJ, Rosalez SE, Wagner BD, Robinson ML
Epigenetics 2017 Jan 2;12(1):27-40
Epigenetics 2017 Jan 2;12(1):27-40
Epigenetic determinants of space radiation-induced cognitive dysfunction.
Acharya MM, Baddour AA, Kawashita T, Allen BD, Syage AR, Nguyen TH, Yoon N, Giedzinski E, Yu L, Parihar VK, Baulch JE
Scientific reports 2017 Feb 21;7:42885
Scientific reports 2017 Feb 21;7:42885
RA-Induced Transcriptional Silencing of Checkpoint Kinase-2 through Promoter Methylation by Dnmt3b Is Required for Neuronal Differentiation of P19 Cells.
Sheikh MA, Malik YS, Zhu X
Journal of molecular biology 2017 Aug 4;429(16):2463-2473
Journal of molecular biology 2017 Aug 4;429(16):2463-2473
Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2.
Thakur A, Mackin SJ, Irwin RE, O'Neill KM, Pollin G, Walsh C
Epigenetics & chromatin 2016;9:53
Epigenetics & chromatin 2016;9:53
Immunohistochemical expression of DNA methyltransferases 1, 3a, and 3b in actinic cheilitis and lip squamous cell carcinomas.
Daniel FI, Alves SR, Vieira DS, Biz MT, Daniel IW, Modolo F
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2016 Nov;45(10):774-779
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2016 Nov;45(10):774-779
Inhibition of TGF-β Signaling Promotes Human Pancreatic β-Cell Replication.
Dhawan S, Dirice E, Kulkarni RN, Bhushan A
Diabetes 2016 May;65(5):1208-18
Diabetes 2016 May;65(5):1208-18
Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway.
Hong B, Su Z, Zhang C, Yang Y, Guo Y, Li W, Kong AN
The AAPS journal 2016 May;18(3):659-69
The AAPS journal 2016 May;18(3):659-69
De novo DNA methylation drives 5hmC accumulation in mouse zygotes.
Amouroux R, Nashun B, Shirane K, Nakagawa S, Hill PW, D'Souza Z, Nakayama M, Matsuda M, Turp A, Ndjetehe E, Encheva V, Kudo NR, Koseki H, Sasaki H, Hajkova P
Nature cell biology 2016 Feb;18(2):225-233
Nature cell biology 2016 Feb;18(2):225-233
Mapping global changes in nuclear cytosine base modifications in the early mouse embryo.
Li Y, Seah MK, O'Neill C
Reproduction (Cambridge, England) 2016 Feb;151(2):83-95
Reproduction (Cambridge, England) 2016 Feb;151(2):83-95
Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells.
Xu X, Tan X, Hulshoff MS, Wilhelmi T, Zeisberg M, Zeisberg EM
FEBS letters 2016 Apr;590(8):1222-33
FEBS letters 2016 Apr;590(8):1222-33
Maternal TET3 is dispensable for embryonic development but is required for neonatal growth.
Tsukada Y, Akiyama T, Nakayama KI
Scientific reports 2015 Oct 28;5:15876
Scientific reports 2015 Oct 28;5:15876
Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1.
Guo Y, Shu L, Zhang C, Su ZY, Kong AN
Biochemical pharmacology 2015 Mar 15;94(2):69-78
Biochemical pharmacology 2015 Mar 15;94(2):69-78
DNA methylation directs functional maturation of pancreatic β cells.
Dhawan S, Tschen SI, Zeng C, Guo T, Hebrok M, Matveyenko A, Bhushan A
The Journal of clinical investigation 2015 Jul 1;125(7):2851-60
The Journal of clinical investigation 2015 Jul 1;125(7):2851-60
DNA methyltransferase immunohistochemical expression in odontogenic tumours.
Guimarães DM, Antunes DM, Duarte CM, Ferro LB, Nunes FD
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2015 Jan;44(1):59-66
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2015 Jan;44(1):59-66
Single-Base Resolution Analysis of 5-Formyl and 5-Carboxyl Cytosine Reveals Promoter DNA Methylation Dynamics.
Neri F, Incarnato D, Krepelova A, Rapelli S, Anselmi F, Parlato C, Medana C, Dal Bello F, Oliviero S
Cell reports 2015 Feb 10;10(5):674-683
Cell reports 2015 Feb 10;10(5):674-683
Deregulation of DNMT1, DNMT3B and miR-29s in Burkitt lymphoma suggests novel contribution for disease pathogenesis.
Robaina MC, Mazzoccoli L, Arruda VO, Reis FR, Apa AG, de Rezende LM, Klumb CE
Experimental and molecular pathology 2015 Apr;98(2):200-7
Experimental and molecular pathology 2015 Apr;98(2):200-7
DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level.
Huichalaf C, Micheloni S, Ferri G, Caccia R, Gabellini D
PloS one 2014;9(12):e115278
PloS one 2014;9(12):e115278
Establishment of a conditional transgenic system using the 2A peptide in the female mouse germline.
Hara S, Takano T, Ogata M, Yamakami R, Sato Y, Kono T, Obata Y
The Journal of reproduction and development 2014;60(3):250-5
The Journal of reproduction and development 2014;60(3):250-5
Establishment of a conditional transgenic system using the 2A peptide in the female mouse germline.
Hara S, Takano T, Ogata M, Yamakami R, Sato Y, Kono T, Obata Y
The Journal of reproduction and development 2014;60(3):250-5
The Journal of reproduction and development 2014;60(3):250-5
Cooperative DNA binding and protein/DNA fiber formation increases the activity of the Dnmt3a DNA methyltransferase.
Emperle M, Rajavelu A, Reinhardt R, Jurkowska RZ, Jeltsch A
The Journal of biological chemistry 2014 Oct 24;289(43):29602-13
The Journal of biological chemistry 2014 Oct 24;289(43):29602-13
Blocking of JB6 cell transformation by tanshinone IIA: epigenetic reactivation of Nrf2 antioxidative stress pathway.
Wang L, Zhang C, Guo Y, Su ZY, Yang Y, Shu L, Kong AN
The AAPS journal 2014 Nov;16(6):1214-25
The AAPS journal 2014 Nov;16(6):1214-25
The combination of the prodrugs perforin-CEBPD and perforin-granzyme B efficiently enhances the activation of caspase signaling and kills prostate cancer.
Chuang CH, Wang WJ, Li CF, Ko CY, Chou YH, Chuu CP, Cheng TL, Wang JM
Cell death & disease 2014 May 8;5:e1220
Cell death & disease 2014 May 8;5:e1220
A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype.
Serra RW, Fang M, Park SM, Hutchinson L, Green MR
eLife 2014 Mar 12;3:e02313
eLife 2014 Mar 12;3:e02313
Apigenin reactivates Nrf2 anti-oxidative stress signaling in mouse skin epidermal JB6 P + cells through epigenetics modifications.
Paredes-Gonzalez X, Fuentes F, Su ZY, Kong AN
The AAPS journal 2014 Jul;16(4):727-35
The AAPS journal 2014 Jul;16(4):727-35
Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting.
Hara S, Takano T, Fujikawa T, Yamada M, Wakai T, Kono T, Obata Y
Human molecular genetics 2014 Jul 15;23(14):3853-64
Human molecular genetics 2014 Jul 15;23(14):3853-64
Long exposure to mature ooplasm can alter DNA methylation at imprinted loci in non-growing oocytes but not in prospermatogonia.
Obata Y, Wakai T, Hara S, Kono T
Reproduction (Cambridge, England) 2014 Jan;147(1):H1-6
Reproduction (Cambridge, England) 2014 Jan;147(1):H1-6
DNMT3B inhibits the re-expression of genes associated with induced pluripotency.
Wongtrakoongate P, Li J, Andrews PW
Experimental cell research 2014 Feb 15;321(2):231-9
Experimental cell research 2014 Feb 15;321(2):231-9
Epigenetic regulation of Dpp6 expression by Dnmt3b and its novel role in the inhibition of RA induced neuronal differentiation of P19 cells.
Sheikh MA, Malik YS, Yu H, Lai M, Wang X, Zhu X
PloS one 2013;8(2):e55826
PloS one 2013;8(2):e55826
miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells.
Hara ES, Ono M, Eguchi T, Kubota S, Pham HT, Sonoyama W, Tajima S, Takigawa M, Calderwood SK, Kuboki T
PloS one 2013;8(12):e83545
PloS one 2013;8(12):e83545
Combined modulation of polycomb and trithorax genes rejuvenates β cell replication.
Zhou JX, Dhawan S, Fu H, Snyder E, Bottino R, Kundu S, Kim SK, Bhushan A
The Journal of clinical investigation 2013 Nov;123(11):4849-58
The Journal of clinical investigation 2013 Nov;123(11):4849-58
Analysis of DNA methylation change induced by Dnmt3b in mouse hepatocytes.
Takahashi M, Kamei Y, Ehara T, Yuan X, Suganami T, Takai-Igarashi T, Hatada I, Ogawa Y
Biochemical and biophysical research communications 2013 May 17;434(4):873-8
Biochemical and biophysical research communications 2013 May 17;434(4):873-8
Naive pluripotency is associated with global DNA hypomethylation.
Leitch HG, McEwen KR, Turp A, Encheva V, Carroll T, Grabole N, Mansfield W, Nashun B, Knezovich JG, Smith A, Surani MA, Hajkova P
Nature structural & molecular biology 2013 Mar;20(3):311-6
Nature structural & molecular biology 2013 Mar;20(3):311-6
Hepatitis B virus inhibits liver regeneration via epigenetic regulation of urokinase-type plasminogen activator.
Park ES, Park YK, Shin CY, Park SH, Ahn SH, Kim DH, Lim KH, Kwon SY, Kim KP, Yang SI, Seong BL, Kim KH
Hepatology (Baltimore, Md.) 2013 Aug;58(2):762-76
Hepatology (Baltimore, Md.) 2013 Aug;58(2):762-76
Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels.
Zimmermann N, Zschocke J, Perisic T, Yu S, Holsboer F, Rein T
The Biochemical journal 2012 Nov 15;448(1):93-102
The Biochemical journal 2012 Nov 15;448(1):93-102
Sodium arsenite represses the expression of myogenin in C2C12 mouse myoblast cells through histone modifications and altered expression of Ezh2, Glp, and Igf-1.
Hong GM, Bain LJ
Toxicology and applied pharmacology 2012 May 1;260(3):250-9
Toxicology and applied pharmacology 2012 May 1;260(3):250-9
Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells.
Stadtfeld M, Apostolou E, Ferrari F, Choi J, Walsh RM, Chen T, Ooi SS, Kim SY, Bestor TH, Shioda T, Park PJ, Hochedlinger K
Nature genetics 2012 Mar 4;44(4):398-405, S1-2
Nature genetics 2012 Mar 4;44(4):398-405, S1-2
Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases.
Selvakumar T, Gjidoda A, Hovde SL, Henry RW
The Journal of biological chemistry 2012 Mar 2;287(10):7039-50
The Journal of biological chemistry 2012 Mar 2;287(10):7039-50
DNA methyltransferases1 (DNMT1) and 3a (DNMT3a) colocalize with GAD67-positive neurons in the GAD67-GFP mouse brain.
Kadriu B, Guidotti A, Chen Y, Grayson DR
The Journal of comparative neurology 2012 Jun 15;520(9):1951-64
The Journal of comparative neurology 2012 Jun 15;520(9):1951-64
Mothers matter too: benefits of temperature oviposition preferences in newts.
Kurdíková V, Smolinský R, Gvoždík L
PloS one 2011;6(8):e23842
PloS one 2011;6(8):e23842
Clinical significance of the expression of DNA methyltransferase proteins in gastric cancer.
Yang J, Wei X, Wu Q, Xu Z, Gu D, Jin Y, Shen Y, Huang H, Fan H, Chen J
Molecular medicine reports 2011 Nov-Dec;4(6):1139-43
Molecular medicine reports 2011 Nov-Dec;4(6):1139-43
De novo DNA methylation by Dnmt3a and Dnmt3b is dispensable for nuclear reprogramming of somatic cells to a pluripotent state.
Pawlak M, Jaenisch R
Genes & development 2011 May 15;25(10):1035-40
Genes & development 2011 May 15;25(10):1035-40
Gene expression and mutation assessment provide clues of genetic and epigenetic mechanisms in liver tumors of oxazepam-exposed mice.
Lahousse SA, Hoenerhoff M, Collins J, Ton TV, Masinde T, Olson D, Rebolloso Y, Koujitani T, Tomer KB, Hong HH, Bucher J, Sills RC
Veterinary pathology 2011 Jul;48(4):875-84
Veterinary pathology 2011 Jul;48(4):875-84
Distinct nuclear localization patterns of DNA methyltransferases in developing and mature mammalian retina.
Nasonkin IO, Lazo K, Hambright D, Brooks M, Fariss R, Swaroop A
The Journal of comparative neurology 2011 Jul 1;519(10):1914-30
The Journal of comparative neurology 2011 Jul 1;519(10):1914-30
DNA methyltransferase 3b preferentially associates with condensed chromatin.
Kashiwagi K, Nimura K, Ura K, Kaneda Y
Nucleic acids research 2011 Feb;39(3):874-88
Nucleic acids research 2011 Feb;39(3):874-88
Epigenetic reprogramming as a key contributor to melanocyte malignant transformation.
Molognoni F, Cruz AT, Meliso FM, Morais AS, Souza CF, Xander P, Bischof JM, Costa FF, Soares MB, Liang G, Jones PA, Jasiulionis MG
Epigenetics 2011 Apr;6(4):450-64
Epigenetics 2011 Apr;6(4):450-64
Epigenetic reprogramming as a key contributor to melanocyte malignant transformation.
Molognoni F, Cruz AT, Meliso FM, Morais AS, Souza CF, Xander P, Bischof JM, Costa FF, Soares MB, Liang G, Jones PA, Jasiulionis MG
Epigenetics 2011 Apr;6(4):450-64
Epigenetics 2011 Apr;6(4):450-64
Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.
Lambrot R, Kimmins S
International journal of andrology 2011 Apr;34(2):110-23
International journal of andrology 2011 Apr;34(2):110-23
DNA cytosine methylation in the bovine leukemia virus promoter is associated with latency in a lymphoma-derived B-cell line: potential involvement of direct inhibition of cAMP-responsive element (CRE)-binding protein/CRE modulator/activation transcription factor binding.
Pierard V, Guiguen A, Colin L, Wijmeersch G, Vanhulle C, Van Driessche B, Dekoninck A, Blazkova J, Cardona C, Merimi M, Vierendeel V, Calomme C, Nguyên TL, Nuttinck M, Twizere JC, Kettmann R, Portetelle D, Burny A, Hirsch I, Rohr O, Van Lint C
The Journal of biological chemistry 2010 Jun 18;285(25):19434-49
The Journal of biological chemistry 2010 Jun 18;285(25):19434-49
Caspase 8 and maspin are downregulated in breast cancer cells due to CpG site promoter methylation.
Wu Y, Alvarez M, Slamon DJ, Koeffler P, Vadgama JV
BMC cancer 2010 Feb 4;10:32
BMC cancer 2010 Feb 4;10:32
A functional polymorphism in the DNA methyltransferase-3A promoter modifies the susceptibility in gastric cancer but not in esophageal carcinoma.
Fan H, Liu D, Qiu X, Qiao F, Wu Q, Su X, Zhang F, Song Y, Zhao Z, Xie W
BMC medicine 2010 Feb 3;8:12
BMC medicine 2010 Feb 3;8:12
Immunohistochemical expression of DNA methyltransferases 1, 3a and 3b in oral leukoplakias and squamous cell carcinomas.
Daniel FI, Rivero ER, Modolo F, Lopes TG, Salum FG
Archives of oral biology 2010 Dec;55(12):1024-30
Archives of oral biology 2010 Dec;55(12):1024-30
Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells.
Meilinger D, Fellinger K, Bultmann S, Rothbauer U, Bonapace IM, Klinkert WE, Spada F, Leonhardt H
EMBO reports 2009 Nov;10(11):1259-64
EMBO reports 2009 Nov;10(11):1259-64
Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres.
May A, Kirchner R, Müller H, Hartmann P, El Hajj N, Tresch A, Zechner U, Mann W, Haaf T
Biology of reproduction 2009 Jan;80(1):194-202
Biology of reproduction 2009 Jan;80(1):194-202
Epigenetic silencing of CCAAT/enhancer-binding protein delta activity by YY1/polycomb group/DNA methyltransferase complex.
Ko CY, Hsu HC, Shen MR, Chang WC, Wang JM
The Journal of biological chemistry 2008 Nov 7;283(45):30919-32
The Journal of biological chemistry 2008 Nov 7;283(45):30919-32
Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation.
Xia G, Schneider-Stock R, Diestel A, Habold C, Krueger S, Roessner A, Naumann M, Lendeckel U
Biochemical and biophysical research communications 2008 May 2;369(2):526-31
Biochemical and biophysical research communications 2008 May 2;369(2):526-31
Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.
Hirasawa R, Chiba H, Kaneda M, Tajima S, Li E, Jaenisch R, Sasaki H
Genes & development 2008 Jun 15;22(12):1607-16
Genes & development 2008 Jun 15;22(12):1607-16
DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation.
Oh BK, Kim H, Park HJ, Shim YH, Choi J, Park C, Park YN
International journal of molecular medicine 2007 Jul;20(1):65-73
International journal of molecular medicine 2007 Jul;20(1):65-73
IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencing.
Zhu F, Xia X, Liu B, Shen J, Hu Y, Person M, Hu Y
Molecular cell 2007 Jul 20;27(2):214-27
Molecular cell 2007 Jul 20;27(2):214-27
Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R
Genes & development 2007 Dec 1;21(23):3110-22
Genes & development 2007 Dec 1;21(23):3110-22
Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein.
Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI
Gastroenterology 2007 Apr;132(4):1476-94
Gastroenterology 2007 Apr;132(4):1476-94
Coordinate regulation of DNA methyltransferase expression during oogenesis.
Lucifero D, La Salle S, Bourc'his D, Martel J, Bestor TH, Trasler JM
BMC developmental biology 2007 Apr 19;7:36
BMC developmental biology 2007 Apr 19;7:36
Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells.
Shen Y, Chow J, Wang Z, Fan G
Human molecular genetics 2006 Sep 1;15(17):2623-35
Human molecular genetics 2006 Sep 1;15(17):2623-35
Dnmt3a2 targets endogenous Dnmt3L to ES cell chromatin and induces regional DNA methylation.
Nimura K, Ishida C, Koriyama H, Hata K, Yamanaka S, Li E, Ura K, Kaneda Y
Genes to cells : devoted to molecular & cellular mechanisms 2006 Oct;11(10):1225-37
Genes to cells : devoted to molecular & cellular mechanisms 2006 Oct;11(10):1225-37
Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body.
Iwatani M, Ikegami K, Kremenska Y, Hattori N, Tanaka S, Yagi S, Shiota K
Stem cells (Dayton, Ohio) 2006 Nov;24(11):2549-56
Stem cells (Dayton, Ohio) 2006 Nov;24(11):2549-56
Roles for Dnmt3b in mammalian development: a mouse model for the ICF syndrome.
Ueda Y, Okano M, Williams C, Chen T, Georgopoulos K, Li E
Development (Cambridge, England) 2006 Mar;133(6):1183-92
Development (Cambridge, England) 2006 Mar;133(6):1183-92
Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development.
Jones B, Chen J
The EMBO journal 2006 Jun 7;25(11):2443-52
The EMBO journal 2006 Jun 7;25(11):2443-52
Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b.
Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, Shimotohno K, Ishikawa F, Li E, Ueda HR, Nakayama J, Okano M
Genes to cells : devoted to molecular & cellular mechanisms 2006 Jul;11(7):805-14
Genes to cells : devoted to molecular & cellular mechanisms 2006 Jul;11(7):805-14
Expression of DNMT3A transcripts and nucleolar localization of DNMT3A protein in human testicular and fibroblast cells suggest a role for de novo DNA methylation in nucleolar inactivation.
Galetzka D, Tralau T, Stein R, Haaf T
Journal of cellular biochemistry 2006 Jul 1;98(4):885-94
Journal of cellular biochemistry 2006 Jul 1;98(4):885-94
DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma.
Park HJ, Yu E, Shim YH
Cancer letters 2006 Feb 28;233(2):271-8
Cancer letters 2006 Feb 28;233(2):271-8
Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse.
La Salle S, Trasler JM
Developmental biology 2006 Aug 1;296(1):71-82
Developmental biology 2006 Aug 1;296(1):71-82
Inhibition of DNA methyltransferase reverses cisplatin induced drug resistance in murine neuroblastoma cells.
Qiu YY, Mirkin BL, Dwivedi RS
Cancer detection and prevention 2005;29(5):456-63
Cancer detection and prevention 2005;29(5):456-63
Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model.
Stempak JM, Sohn KJ, Chiang EP, Shane B, Kim YI
Carcinogenesis 2005 May;26(5):981-90
Carcinogenesis 2005 May;26(5):981-90
Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system.
Feng J, Chang H, Li E, Fan G
Journal of neuroscience research 2005 Mar 15;79(6):734-46
Journal of neuroscience research 2005 Mar 15;79(6):734-46
Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development.
Park IY, Sohn BH, Choo JH, Joe CO, Seong JK, Lee YI, Chung JH
Journal of cellular biochemistry 2005 Feb 15;94(3):585-96
Journal of cellular biochemistry 2005 Feb 15;94(3):585-96
Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development.
MacDonald JL, Gin CS, Roskams AJ
Developmental biology 2005 Dec 15;288(2):461-73
Developmental biology 2005 Dec 15;288(2):461-73
De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine.
Oka M, Meacham AM, Hamazaki T, Rodić N, Chang LJ, Terada N
Oncogene 2005 Apr 28;24(19):3091-9
Oncogene 2005 Apr 28;24(19):3091-9
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer.
Leu YW, Yan PS, Fan M, Jin VX, Liu JC, Curran EM, Welshons WV, Wei SH, Davuluri RV, Plass C, Nephew KP, Huang TH
Cancer research 2004 Nov 15;64(22):8184-92
Cancer research 2004 Nov 15;64(22):8184-92
Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation.
Weisenberger DJ, Velicescu M, Cheng JC, Gonzales FA, Liang G, Jones PA
Molecular cancer research : MCR 2004 Jan;2(1):62-72
Molecular cancer research : MCR 2004 Jan;2(1):62-72
Expression of DNA methyltransferases in multistep hepatocarcinogenesis.
Choi MS, Shim YH, Hwa JY, Lee SK, Ro JY, Kim JS, Yu E
Human pathology 2003 Jan;34(1):11-7
Human pathology 2003 Jan;34(1):11-7
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin.
Yan Q, Cho E, Lockett S, Muegge K
Molecular and cellular biology 2003 Dec;23(23):8416-28
Molecular and cellular biology 2003 Dec;23(23):8416-28
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin.
Yan Q, Cho E, Lockett S, Muegge K
Molecular and cellular biology 2003 Dec;23(23):8416-28
Molecular and cellular biology 2003 Dec;23(23):8416-28
Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b.
Chen T, Ueda Y, Dodge JE, Wang Z, Li E
Molecular and cellular biology 2003 Aug;23(16):5594-605
Molecular and cellular biology 2003 Aug;23(16):5594-605
Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b.
Chen T, Ueda Y, Dodge JE, Wang Z, Li E
Molecular and cellular biology 2003 Aug;23(16):5594-605
Molecular and cellular biology 2003 Aug;23(16):5594-605
A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation.
Chen T, Ueda Y, Xie S, Li E
The Journal of biological chemistry 2002 Oct 11;277(41):38746-54
The Journal of biological chemistry 2002 Oct 11;277(41):38746-54
Lsh, a member of the SNF2 family, is required for genome-wide methylation.
Dennis K, Fan T, Geiman T, Yan Q, Muegge K
Genes & development 2001 Nov 15;15(22):2940-4
Genes & development 2001 Nov 15;15(22):2940-4
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Supportive validation
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- Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was probed with 2 ug/mL anti-Dnmt3a in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the decrease in Dnmt3a expression upon treatment with 500 uM Zebularine (arrowhead). Additional bands at 90 and 75 kDa can also be detected with this antibody and may represent alternative splice variants. Tubulin is shown as a loading control.
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- Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL.
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- Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - 1 ug of recombinant purified DNMT3A protein. Image from verified customer review.
Supportive validation
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- Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - Detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). The antibody generated a specific cytoplasmic staining in all the cancer cells while some of the cells depicted nuclear staining also.
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- Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - FFPE human liver stained with Dnmt3a antibody at 5 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
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- Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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- Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Using the PE direct conjugate, an intracellular stain was performed on NTERA-2 cells with DNMT3A (64B1446) NB120-13888PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
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- Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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- Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).
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- Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on Jurkat cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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- Main image
- Experimental details
- Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on Jurkat cells with DNMT3A Antibody (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
