Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-35052 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V1B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, Drosophila, C. elegans, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Loss of Adam10 Disrupts Ion Transport in Immortalized Kidney Collecting Duct Cells.
mCCD(cl1) cells show plasticity consistent with the ability to transition between principal and intercalated cells.
Assmus A, Mullins L, Ward M, Dobie R, Hunter R, Henderson NC, Mullins JJ
Function (Oxford, England) 2021;2(4):zqab024
Function (Oxford, England) 2021;2(4):zqab024
mCCD(cl1) cells show plasticity consistent with the ability to transition between principal and intercalated cells.
Assmus AM, Mansley MK, Mullins LJ, Peter A, Mullins JJ
American journal of physiology. Renal physiology 2018 May 1;314(5):F820-F831
American journal of physiology. Renal physiology 2018 May 1;314(5):F820-F831
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP6V1B1 in WiDr and K562 cell lysates (35 µg/lane) using an ATP6V1B1 polyclonal antibody (Product # PA5-35052).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATP6V1B1 in WiDr cells using an ATP6V1B1 polyclonal antibody (Product # PA5-35052) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ATP6V1B1 in formalin-fixed, paraffin-embedded human kidney tissue using an ATP6V1B1 polyclonal antibody (Product # PA5-35052) followed by peroxidase conjugation of the secondary antibody and DAB staining.